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  • by Hinnerk Boriss
  • Published  


The TRANSIL HSA Binding Kit estimates the binding of drugs to human serum albumin (HSA) and predicts the plasma protein binding. The assay kit measures the dissociation constant (KD) of drugs to albumin and hence allows the calculation of albumin binding even under disease and physiological states that alter the albumin content of human serum. In combination with our TRANSIL AGP Binding Kit it is possible to obtain accurate prediction of plasma protein binding in a highly controlled and reproducible assay environment. Internal quality controls provide easy assessment of recovery, experiment and data quality.

TRANSIL protein binding kits measure the dissociation constant (KD) of the test item in 6 intelligent replicates with varying protein concentration. This allows not only accurate KD determination, but also provides for detailed assay quality control, so that there is no need to perform additional experiments to assess recovery and concentration dependency. We also provide a spreadsheet for the data analysis and comprehensive quality control. The automatically calculates the test items’ dissociation constants and the fraction unbound according to this formula:

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